Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Neural

Cell type

Cerebellar Vermis

MeSH Description

A subregion of the CEREBELLAR CORTEX, located along the midsagittal plane of the CEREBELLUM.

Sample

source_name

anterior dorsal cerebellar vermis

genotype

Ai32 fl/fl

developmental stage

6-8 weeks

tissue

cerebellum

chip antibody

H3K27ac

chip antibody vendor

Abcam

behavior

homecage

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cerebella were removed and microdissected. For ChIP, lysates were purified from sonicated nuclei and histone-DNA complexes were isolated with the indicated antibodies. For HiC and PLAC-Seq, lysates of nuclei digested with MboI and re-ligated with biotin fill-in were sonicated and subjected to ChIP (PLAC) and biotin-IP. RNA was fractionated and harvested with Trizol. For DHS, nuclei were digested with DNase I and DNA fragments smaller than 500bp were purified. ChIP-Seq, HiC, and PLAC-Seq libraries were prepared with the NEBNext Ultra™ II DNA Library Prep Kit. RNA libraries were prepared with the NEBNext Ultra™ Directional RNA Library Prep Kit. DHS libraries were prepared with the Illumina DNA Sample Prep Kit.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

24853568

Reads aligned (%)

92.3

Duplicates removed (%)

13.5

Number of peaks

29471 (qval < 1E-05)

mm9

Number of total reads

24853568

Reads aligned (%)

92.2

Duplicates removed (%)

13.6

Number of peaks

29459 (qval < 1E-05)