Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NPM1

Cell type

Cell type Class
Blood
Cell type
OCI-AML3
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
OCI-AML3
cell type
human leukemia
cell line
OCI-AML3
chip antibody
anti-NPM1(mutant)(ThermoFischer, PA1-46356)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cross-linked chromatin was sheared by sonication, and MNase treatment. To repair ChIP-DNA and Input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with Illumina (PCR primer InPE1.0 & InPE2.0).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
26386895
Reads aligned (%)
12.7
Duplicates removed (%)
87.1
Number of peaks
113 (qval < 1E-05)

hg38

Number of total reads
26386895
Reads aligned (%)
13.6
Duplicates removed (%)
86.2
Number of peaks
86 (qval < 1E-05)

Base call quality data from DBCLS SRA