Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse ES
cell type
mouse ES
strain
129/Ola
chip antibody
FLAG (SIGMA, F1804)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cross-linked chromatin was sheared by sonication, and MNase treatment. To repair ChIP-DNA and Input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with Illumina (PCR primer InPE1.0 & InPE2.0).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
31432716
Reads aligned (%)
97.1
Duplicates removed (%)
63.2
Number of peaks
217 (qval < 1E-05)

mm9

Number of total reads
31432716
Reads aligned (%)
96.9
Duplicates removed (%)
63.3
Number of peaks
229 (qval < 1E-05)

Base call quality data from DBCLS SRA