ChIP-Atlas: SRX5487475

Antigen

Cell type Class: Blood
Cell type: Bone Marrow Cells
MeSH Description: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: RNA-seq: Total RNA was isolated from the FACS sorted CAR and PaS cells using RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. The total mRNA was enriched by using Oligo dT magnetic beads. ATAC-seq: minimum of 10.000 cells were resuspended in 50 µL of cold lysis buffer (10 mM Tris·Cl at pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% [v/v] Igepal CA-630) and incubated for 15 min on ice. The supernatant was discarded after centrifugation, and the nuclei were used for transposition reaction using NEB Nextera DNA sample preparation kit. The RNA-seq libraries were prepared by using SMART-Seq v4 ultra-low input RNA library preparation protocol according to the manufacturer's instructions. The ATAC-seq libraries were prepared using the NEB Nextera DNA sample preparation kit according to the manufacturer's instructions.