GSM3640243: WP HA-hERa AdCre aHA repl1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Breast
Cell type
Mammary epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Primary mouse mammary epithelial cells
strain
FVB
cell type
Primary MMECs
genotype
WcrH;P53F/F;HA-hERa
chip antibody
aHA (Millipore, catalog# 05-904)
treatment
AdCre transduction, harvested after 72hrs
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect the HA-tag (25ug, 05-904, Millipore) and ERa (10ug, sc-543, Santa Cruz) with 100 ml Protein A magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit).