SRX5463248
GSM3639157: P213JAEKO mESC H3K27me3 ChIP replicate1; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
strain
129B6F1
chip antibody
H3K27me3 (Cell Signaling, Rabbit mAb, C36B11)
genotype
Pcl1-3 + Jarid2 + Aebp2 + Epop KO
experiment type
ChIP-Seq

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitation of formaldehyde cross-linked and sonicated cell lysate. NEBNext Ultra II protocol (NEB, E7645S)

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
22420484
Reads aligned (%)
94.5
Duplicates removed (%)
8.9
Number of peaks
177 (qval < 1E-05)

mm9

Number of total reads
22420484
Reads aligned (%)
94.5
Duplicates removed (%)
9.0
Number of peaks
164 (qval < 1E-05)

Base call quality data from DBCLS SRA