Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
0-6h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
0-6 hr old Oregon R embryos
developmental stage
0-6 hr old embryo
antibodies
H3K27me3 (Cell Signaling (C36B11))

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq kit.

Sequencing Platform

instrument_model
NextSeq 500

dm6

Number of total reads
18541787
Reads aligned (%)
87.7
Duplicates removed (%)
12.1
Number of peaks
2573 (qval < 1E-05)

dm3

Number of total reads
18541787
Reads aligned (%)
87.9
Duplicates removed (%)
12.0
Number of peaks
2636 (qval < 1E-05)

Base call quality data from DBCLS SRA