Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kdm4a

Cell type

Cell type Class
Embryonic fibroblast
Cell type
3T3-L1
Tissue
Embryo
Cell Type
Fibroblast

Attributes by original data submitter

Sample

source_name
3T3-L1
cell line
3T3-L1
transfected with
siRNAs specific for GPS2
chip antibody
anti-KMD4A antibody
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as described (Perissi et al. 2004). The anti-KMD4A antibody (Abcam ab47984) was used. Extracted DNA was ligated to specific adaptors followed by deep sequencing on the Illumina GAII system according to the manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
19906992
Reads aligned (%)
85.8
Duplicates removed (%)
11.1
Number of peaks
441 (qval < 1E-05)

mm9

Number of total reads
19906992
Reads aligned (%)
85.5
Duplicates removed (%)
11.1
Number of peaks
507 (qval < 1E-05)

Base call quality data from DBCLS SRA