Antigen

Antigen Class

Histone

Antigen

H3K9K14ac

Cell type

Cell type Class

Neural

Cell type

Cerebellum

MeSH Description

The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.

Sample

source_name

H3K9/14ac WT P22

genotype/variation

WT

developmental stage

Postnatal day 22

tissue

cerebellum

chip antibody

H3K9/14ac

chip antibody vendor

Millipore

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cerebella were cross-linked and lysed in RIPA buffer. After sonication, immunoprecipitation reactions were performed with antibodies. Libraries prepared with the Illumina ChIP-Seq DNA Sample Prep Kit as per the manufacturer’s instructions and sequenced on the Illumina HiSeq 2000 platform (Beijing Genomics Institute)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

22126564

Reads aligned (%)

95.3

Duplicates removed (%)

7.1

Number of peaks

23185 (qval < 1E-05)

mm9

Number of total reads

22126564

Reads aligned (%)

95.3

Duplicates removed (%)

7.1

Number of peaks

23192 (qval < 1E-05)