Antigen

Antigen Class

TFs and others

Antigen

KLF4

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

human epidermal keratinocytes

cell type

human epidermal keratinocytes

tissue

primary human epidermal keratinocytes isolate from foreskin

chip antibody

R&D AF3640 Lot # XVH0311061

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The isolated DNA was purified using Qiagen columns. We used the NuGen Mondrian for library preparation for Illumina sequencing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

31508997

Reads aligned (%)

91.6

Duplicates removed (%)

18.1

Number of peaks

15188 (qval < 1E-05)

hg19

Number of total reads

31508997

Reads aligned (%)

91.0

Duplicates removed (%)

19.2

Number of peaks

15151 (qval < 1E-05)