Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KLF4

Cell type

Cell type Class
Epidermis
Cell type
Keratinocytes
MeSH Description
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Attributes by original data submitter

Sample

source_name
human epidermal keratinocytes
cell type
human epidermal keratinocytes
tissue
primary human epidermal keratinocytes isolate from foreskin
chip antibody
R&D AF3640 Lot # XVH0311061

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The isolated DNA was purified using Qiagen columns. We used the NuGen Mondrian for library preparation for Illumina sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
31508997
Reads aligned (%)
73.5
Duplicates removed (%)
19.2
Number of peaks
15151 (qval < 1E-05)

hg38

Number of total reads
31508997
Reads aligned (%)
75.0
Duplicates removed (%)
18.1
Number of peaks
15188 (qval < 1E-05)

Base call quality data from DBCLS SRA