Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

dmCas9-ChIPseq No trgRNA IP

cell line

Arf −/− MEFs

cell type

mouse embryonic fibroblasts

antibody

FLAG antibody F1804, SIGMA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Protein-DNA complexes were immunoprecipitated from sonicated chromatin extract with anti-FLAG antibody. NEBNext ChIP-Seq Library Prep Reagent Set for Illumina (E6200S, NEB) according to the manufacturer's protocol. For BARCODE ChIP-seq library we used the BARCODE adaptor for generating the library (BARCODE adaptor: PMID 19159457).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

34153745

Reads aligned (%)

91.5

Duplicates removed (%)

48.4

Number of peaks

673 (qval < 1E-05)

mm9

Number of total reads

34153745

Reads aligned (%)

91.3

Duplicates removed (%)

48.5

Number of peaks

738 (qval < 1E-05)