Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Th17 Cells

MeSH Description

A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Sample

source_name

Th17

treatment

DMSO

donor

2

antibody

H3K27me3

cell type

Th17 cell

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

33888127

Reads aligned (%)

89.5

Duplicates removed (%)

1.2

Number of peaks

816 (qval < 1E-05)

hg19

Number of total reads

33888127

Reads aligned (%)

88.8

Duplicates removed (%)

1.3

Number of peaks

674 (qval < 1E-05)