GSM3619223: ATAC (CD8/Ly5.1) TCR OT-I CD8 T cells isolated D20-21 from malignant liver lesions replicate#2; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Spleen
Cell type
Splenocytes
NA
NA
Attributes by original data submitter
Sample
source_name
mouse splenocytes
strain
C57BL/6
cd8 t cells
TCR OT-I transgenic CD8 T cells
biological context
malignant liver lesions
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
CD8 T cells were FACS-sorted into FCS containing tubes and after sort 10% DMSO were added, mixed and stored at -80°C until all samples were collected. Profiling of Chromatin was performed by ATAC-seq. Cells were thawed and washed in cold PBS and lysed. After cell lysis, transposition was performed at 42ºC for 45 min. After purification of the DNA with the MinElute PCR purification kit (Qiagen), material was amplified for 5 cycles. Additional PCR cycles were evaluated by real time PCR. Final product was cleaned by Ampure Beads at a 1.5x ratio. Libraries were sequenced on a Hiseq 2500 1T in a 50bp/50bp Paired end run, using the TruSeq SBS Kit v3 (Illumina).