Antigen

Antigen Class

TFs and others

Antigen

NETO2

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

human AML cells

cell line

Kasumi-1

cell type

human t(8;21) AML cells

chip antibody

ETO2 (Santa Cruz, SC-9741)

transduced with

lentiviruses expressing ETO2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads, Cell Signaling Technology #9003) following the manufacturer's instructions. Libraries were constructed following instructions accompanying the library preparation kit designed for Illumina sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

36479890

Reads aligned (%)

98.2

Duplicates removed (%)

30.2

Number of peaks

869 (qval < 1E-05)

hg19

Number of total reads

36479890

Reads aligned (%)

97.2

Duplicates removed (%)

31.3

Number of peaks

866 (qval < 1E-05)