Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mouse embryonic stem cells

tissue

mESC in serum, transfected with appropriate plasmid

transfected with

Dppa4_FL

chip antibody

anti-HA.11 (Covance)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP and library preparation were done according to (De Iaco 2017) Libraries were ligated with Illumina adaptors

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

20770202

Reads aligned (%)

95.1

Duplicates removed (%)

16.9

Number of peaks

3164 (qval < 1E-05)

mm9

Number of total reads

20770202

Reads aligned (%)

95.0

Duplicates removed (%)

17.1

Number of peaks

3054 (qval < 1E-05)