GSM1385043: R bulk Dnmt3a2 ChIPSeq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Dnmt TKO ESCs
cell type
ESC
construct
R bulk Dnmt3a2
passages
7 to 10
chip antibody
FLAG (Sigma, F1804)
cell line
J1 ES
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from formaldehyde fixed cell (2x107 cells per ChIP) was fragmented to a size range of 160-480 bp using a combination of micrococcal nuclease digestion and sonication with Bioruptor (Diagenode). Solubilized chromatin was immunoprecipitated with antibody. To generate libraries for Illumina high-throughput sequencing of ChIP and input DNA, end repair, adenylation, ligation with indexed adaptors, and 18 cycles of PCR with Illumina primers were performed following the TruSeq ChIP Sample Preparation guide (Illumina).