Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
BRD3 H1 human Embryonic stem cells (hESC)
cell type
H1 human Embryonic stem cells (hESC)
state
Differentiated in RPMI, B27 supplement, and 50 ng/mL activin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
BRD3 ChIP was pefromed using an anti-BRD3 (Bethyl A302-368A) ChIP-seq libraries were prepared according to instructions accompanying the NEBNext Ultra DNA Library Prep Kit for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
22723246
Reads aligned (%)
91.8
Duplicates removed (%)
5.9
Number of peaks
4360 (qval < 1E-05)

hg38

Number of total reads
22723246
Reads aligned (%)
93.7
Duplicates removed (%)
4.6
Number of peaks
4472 (qval < 1E-05)

Base call quality data from DBCLS SRA