Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

cultured mESC (V6.5)

strain

C57BL/6

cell type

cultured mESC (V6.5)

fixed by

formaldehyde

molecule subtype

ChIP-DNA

ip reagent

bio-ChIP with SA beads

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked cells were treated with 0.3% SDS at 37°C and followed by gentle sonication. Transposition was set up by incubation at 37°C for 1 h, followed by addition of stopping buffer. Soluble chromatin was used for following ChIP experiments. DNA was eluted from beads, reverse crosslinked and treated with proteinase K. Finally, DNA was extracted by pheno-chloroform. After PCR enrichment using NEBNext Q5 Ultra II master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

19068303

Reads aligned (%)

92.2

Duplicates removed (%)

84.3

Number of peaks

103 (qval < 1E-05)

mm9

Number of total reads

19068303

Reads aligned (%)

92.1

Duplicates removed (%)

84.3

Number of peaks

80 (qval < 1E-05)