an early T leukemia cell line that is derived from Ikaros knock-out mice
cell treatment
DN3 cells transduced with Ikaros for 1 day
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Approximately 50k cells for each sample were collected, washed using PBS and lysed using cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Isolated nuclei were incubated with transposition reaction mix at 37℃ for 30 min. DNA was purified using Qiagen MinElute Kit immediately following transposition. Transposed DNA fragments were amplified by PCR for the number of cycles required to obtain 20-40nM of DNA. Barcode primers Ad2.1, Ad2.2, Ad2.3 and Ad2.4 were used for DN3-wt, DN3 (IK)-day 1, DN3 (IK)-day 2 and DN3 (IK)-day 3, respectively. Amplified libraries were then purified by Agencourt AMPure XP beads and QC was performed by BioAnalyzer.