Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
JE131
NA
NA

Attributes by original data submitter

Sample

source_name
JE131 cell line (DN3 cells)
cell line
DN3
cell type
an early T leukemia cell line that is derived from Ikaros knock-out mice
cell treatment
DN3 cells transduced with Ikaros for 2 days
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to instructions for KAPA HyperPrep kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
45480665
Reads aligned (%)
95.1
Duplicates removed (%)
15.8
Number of peaks
635 (qval < 1E-05)

mm9

Number of total reads
45480665
Reads aligned (%)
94.8
Duplicates removed (%)
15.8
Number of peaks
763 (qval < 1E-05)

Base call quality data from DBCLS SRA