Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Pancreas

Cell type

INS-1E

Primary Tissue

Pancreas

Tissue Diagnosis

Carcinoma

Sample

source_name

RNA PolII_ChIPseq

cell line

INS-1

cell type

insulinoma cells

chip antibody

rabbit anti-RPB1 (Cell Signaling 2629)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody ChIP-seq libraries were constructed with NEBNext ChIPseq library kit (NEB) according to manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

rn6

Number of total reads

26526489

Reads aligned (%)

97.3

Duplicates removed (%)

18.4

Number of peaks

20301 (qval < 1E-05)