Antigen

Antigen Class

TFs and others

Antigen

Creb1

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

CREB_ChIPseq

cell type

cultured mouse primary hepatocytes

sample treatment

1 hour 10 uM forskolin

chip antibody

244 anti-CREB rabbit serum

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody ChIP-seq libraries were constructed with NEBNext ChIPseq library kit (NEB) according to manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

25621167

Reads aligned (%)

28.5

Duplicates removed (%)

10.1

Number of peaks

1527 (qval < 1E-05)

mm9

Number of total reads

25621167

Reads aligned (%)

28.4

Duplicates removed (%)

10.0

Number of peaks

1531 (qval < 1E-05)