Antigen

Antigen Class

TFs and others

Antigen

Creb1

Cell type

Cell type Class

Pancreas

Cell type

Islets of Langerhans

MeSH Description

Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.

Sample

source_name

CREB_ChIPseq

cell type

cultured mouse primary pancreatic islets

chip antibody

244 anti-CREB rabbit serum

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody ChIP-seq libraries were constructed with NEBNext ChIPseq library kit (NEB) according to manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

19514322

Reads aligned (%)

65.7

Duplicates removed (%)

11.1

Number of peaks

6905 (qval < 1E-05)

mm9

Number of total reads

19514322

Reads aligned (%)

65.5

Duplicates removed (%)

11.2

Number of peaks

6905 (qval < 1E-05)