GSM3603255: ATAC-Seq - NeuN positive/TDP-43 positive S4; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Neural
Cell type
Frontal cortex
NA
NA
Attributes by original data submitter
Sample
source_name
Nuclei
tissue
Frontal cortex
gender
female
diagnosis
FTD
genotype/variation
TDP-43 positive
cell type
NeuN positive
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Nuclei were extracted from frozen post-mortem frontal cortex and neuronal nuclei populations were obtained by FACS. ATAC-seq libraries were created as described in Buenrostro et al. 2015. Briefly, cells were lysed and treated with Tn5 Transposase from the Nextera Library Prep kit from Illumina for 30 min at 37°C. DNA was purified with Qiagen MinElute PCR purification kit and libraries were generated by PCR amplification using Nextera Index Primers from Illumina.