Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embrionic stem cell
cell type
embryonic stem cell
chip antibody
anti-HA home made

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1% formaldehyde cross-linked chromatin was sheared by sonication and the DNA fragments associated with the proteins of interest were selectively immunoprecipitated using specific antibodies Libraries were prepared according to Blecher-Gonen R., et al. 2013 and sequenced on Illumina HiSeq 2000 Chip-Seq, ATAC-Seq, SMART-Seq

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

mm10

Number of total reads
46598798
Reads aligned (%)
96.7
Duplicates removed (%)
22.0
Number of peaks
712 (qval < 1E-05)

mm9

Number of total reads
46598798
Reads aligned (%)
96.5
Duplicates removed (%)
21.9
Number of peaks
778 (qval < 1E-05)

Base call quality data from DBCLS SRA