GSM3597646: ATAC-seq human primary fetal astrocytes batch 2 rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Neural
Cell type
Fetal astrocytes
NA
NA
Attributes by original data submitter
Sample
source_name
ATAC-seq_human primary fetal astrocytes batch 2
cell type
human primary fetal astrocytes batch 2
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were harvested, snap frozen in dry ice, and stored at -80C. Cells were washed with ice cold PBS containing protease inhibitor and nuclei were extracted prior to library construction. The standard ATAC-seq protocol was used, with the transposase reaction carried out for 30 minutes at 37C. 8-10 cycles of amplification were performed and dual size selection was performed with Ampure XP beads.