Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
BJ ATCC ® CRL-2522™
tissue
skin; foreskin normal
growth
cell culture, 37˚C
vector
p63

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATACseq experiments were performed as previously described (Buenrostro et al) using 100,000 cells and 5µL Tn5 transposase (Nextera XT Kit, Illumina) to tagment intact nuclei. ATACseq experiments were performed as previously described (Buenrostro et al) using 100,000 cells and 5µL Tn5 transposase (Nextera XT Kit, Illumina) to tagment intact nuclei.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
34323317
Reads aligned (%)
94.9
Duplicates removed (%)
14.9
Number of peaks
38448 (qval < 1E-05)

hg19

Number of total reads
34323317
Reads aligned (%)
94.4
Duplicates removed (%)
15.1
Number of peaks
38296 (qval < 1E-05)

Base call quality data from DBCLS SRA