ChIP-Atlas: SRX5358161

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: RNA isolation was performed using TRIzol (Invitrogen), two sequential chloroform extractions, Glycoblue carrier (Thermo Fisher), isopropanol precipitation, and washes with 75% ethanol. For RNA-seq, cDNA libraries were constructed and amplified using the SMART-Seq v4 ultra low RNA prep (samples 1-6, 19-28) or the SMARTer Stranded Total RNA-Seq Kit (v2) - Pico Input Mammalian (samples 7-12) per the manufacturer's instructions. Libraries were amplified and then sequenced on an Illumina NextSeq (2 x 75, paired-end). For ATAC-seq, libraries were prepared using the protocol described by Chen et al, Nat. Methods 13, 1013–1020 (2016) and then sequenced on an Illumina NextSeq (2 x 75, paired-end).