Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
Naive B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Pol2_WT_primary B-cells
strain background
C57BL/6
genotype/variation
WT
age
7-10 weeks
tissue
spleen
cell type
primary naive mouse B-cells
treatment
untreated
chip antibody
RNAPII (Santa Cruz sc899)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 250 bp were immunoprecipitated using different antibodies. Libraries were prepared for Illumina sequencing using a standard protocol consisting in blunting, addition of dA overhangs, ligation of Illumina adapters, selection with AMPure beads and PCR with index primers.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
71827062
Reads aligned (%)
77.7
Duplicates removed (%)
24.4
Number of peaks
10267 (qval < 1E-05)

mm9

Number of total reads
71827062
Reads aligned (%)
77.4
Duplicates removed (%)
24.3
Number of peaks
10304 (qval < 1E-05)

Base call quality data from DBCLS SRA