SRX5353203
GSM3596231: 3k16-dox NEBNext Ultra II and NEBNext Multiplex Oligos; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
healthy control iPSC cell line 2242-1 with TRE-H3.3K27M pB construct, subclone B
treatment
Day 14 NPCs (d14), Day 44 eOPCs (d44)
antibody
H3K27Ac (Active Motif #39133), H3K27me3 (Active Motif #39155)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
135182614
Reads aligned (%)
96.6
Duplicates removed (%)
9.9
Number of peaks
19967 (qval < 1E-05)

hg19

Number of total reads
135182614
Reads aligned (%)
95.9
Duplicates removed (%)
10.3
Number of peaks
17936 (qval < 1E-05)

Base call quality data from DBCLS SRA