Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
healthy control iPSC cell line 2242-1 with TRE-H3.3K27M pB construct, subclone A
treatment
Day 14 NPCs (d14), Day 44 eOPCs (d44)
antibody
H3K27Ac (Active Motif #39133), H3K27me3 (Active Motif #39155)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
166138870
Reads aligned (%)
96.5
Duplicates removed (%)
16.5
Number of peaks
23882 (qval < 1E-05)

hg19

Number of total reads
166138870
Reads aligned (%)
95.7
Duplicates removed (%)
17.0
Number of peaks
21436 (qval < 1E-05)

Base call quality data from DBCLS SRA