Nuclei of MLE12 cells were disrupted and sonicated to yield fragment size ranging from 200-500bp. Lysates were incubated with indicated primary antibody, bound DNA was purified using Qiagen mini elute PCR purification kit. Clontech DNA SMART™ ChIP-Seq Kit Sequencing on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in minimum of 20M reads per library with 1x75bp single end setup.