Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

LoVo

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

CRC cell line

sample type

MLH1-/- COLO-205 subclones

chip antibody

rabbit anti-H3K27ac (Abcam #4729).

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq libraries were prepared with NEB reagents as previously described. Briefly, DNA was end-repaired and the blunt, phosphorylated ends were treated to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers and library fragments of ~250 bp were band isolated from an agarose gel.

Sequencing Platform

instrument_model

NextSeq 550

hg38

Number of total reads

61386974

Reads aligned (%)

88.6

Duplicates removed (%)

9.0

Number of peaks

51736 (qval < 1E-05)

hg19

Number of total reads

61386974

Reads aligned (%)

88.3

Duplicates removed (%)

9.3

Number of peaks

51591 (qval < 1E-05)