Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Myeloid Progenitor Cells

MeSH Description

Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.

Sample

source_name

Myeloid Progenitor Cells

strain

C57BL/6

genotype

WT

tissue

Bone Marrow

cell type

Myeloid Progenitor Cells

treatment

untreated

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lin-cKIT+Sca-1- myeloid progenitor cells were isolated from the BM of experimental mice by FACS. Nuclei were isolated from cross-linked cells and sonicated to shear chromatin, then histone-DNA complexes were immunoprecipitated using relevant antibodies. DNA libraries were prepared using the KAPA Hyper Prep Kit for Illuminaplatforms (Kapa Biosystems) and the SeqCap Adapter Kit (Roche) following vendor's instructions.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

24426895

Reads aligned (%)

97.0

Duplicates removed (%)

12.3

Number of peaks

346 (qval < 1E-05)

mm9

Number of total reads

24426895

Reads aligned (%)

96.8

Duplicates removed (%)

12.4

Number of peaks

336 (qval < 1E-05)