GSM3592591: H3K4me1 noDox V; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Gonad
Cell type
SVOG-3e
NA
NA
Attributes by original data submitter
Sample
source_name
SVOG3e cells empty vector control
cell type
SV40 large T antigen immortalized human granulosa cells
genotype/variation
empty vector control
induction
non-induced
antibody
anti-H3K4me1, Diagenode, C15410037
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were trypsinized and collected by spin at 300g for 5 min. Then cell pellets were snap-freezed We performed native chromatin immunoprecipitation (ChIP) as described previously (Lorzadeh et al., 2016). Libraries were constructed using an Agilent Bravo automated liquid handling platform. Illumina sequencing libraries were generated by end repair, 3' A-addition, and Illumina sequencing adaptor ligation.