SV40 large T antigen immortalized human granulosa cells
genotype/variation
empty vector control
induction
12 hours Doxycycline induction
antibody
anti-H3K27ac, Hiroshi Kimura, Kimura et al. 2008 PMID: 18227620
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were trypsinized and collected by spin at 300g for 5 min. Then cell pellets were snap-freezed We performed native chromatin immunoprecipitation (ChIP) as described previously (Lorzadeh et al., 2016). Libraries were constructed using an Agilent Bravo automated liquid handling platform. Illumina sequencing libraries were generated by end repair, 3' A-addition, and Illumina sequencing adaptor ligation.