GSM3592275: CTRtetCASZ1b H3K27me3 Ctrl; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Muscle
Cell type
SMS-CTR
NA
NA
Attributes by original data submitter
Sample
source_name
SMS-CTR cells
cell type
Rhabdomyosarcoma
genotype
stably transfected with CASZ1b, Dox inducible
dox status
No dox
chip antibody
H3K27me3 (Millipore, 07-449)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.