Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Colorectal cancer cells
cell type
HCT116
chip antibody
p53 (Santa Cruz sc-126)
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed by VATHS Universal DNA Library Prep Kit for Illumina (Vazyme ND604). 

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
14050352
Reads aligned (%)
135.2
Duplicates removed (%)
8.5
Number of peaks
192 (qval < 1E-05)

hg38

Number of total reads
14050352
Reads aligned (%)
137.1
Duplicates removed (%)
8.3
Number of peaks
376 (qval < 1E-05)

Base call quality data from DBCLS SRA