Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

PBMC

Tissue

blood

Lineage

mesoderm

Description

peripheral blood mononuclear cells

Sample

source_name

PBMCs

time

48

treatment_code

HIV-GFP-CD86hi

donor_code

D183

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Nuclei from 50,000 sorted DCs were subjected to a transposase reaction as described in Buenrostro, 2013. Sequencing libraries were constructed using a modified version of the Illumina Nextera DNA Sample prep kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

21260206

Reads aligned (%)

77.3

Duplicates removed (%)

65.3

Number of peaks

5135 (qval < 1E-05)

hg19

Number of total reads

21260206

Reads aligned (%)

77.1

Duplicates removed (%)

65.3

Number of peaks

5139 (qval < 1E-05)