Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Prostate

Cell type

PC-3M

NA

NA

Sample

source_name

PC-3M cells

differentiation stage

prostate cancer cells

genotype

AR-independent

passages

11

chip antibody

anti-Pol II CTD S5ph, #C15200007, lot 001-12, Diagenode

sirna

Ctrl

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Metzger, E., et al. Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation. Nat Cell Biol 10, 53-60 (2008). Libraries were prepared from immunoprecipitated DNA according to standard methods. ChIP-seq libraries were sequenced using a HiSeq 2000 (Illumina) and mapped to the hg19 reference genome using STAR

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

10105883

Reads aligned (%)

41.3

Duplicates removed (%)

18.3

Number of peaks

1161 (qval < 1E-05)

hg19

Number of total reads

10105883

Reads aligned (%)

41.1

Duplicates removed (%)

18.4

Number of peaks

1156 (qval < 1E-05)