GSM3583893: ChIP polII siRNA Ctrl PC-3M; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Prostate
Cell type
PC-3M
NA
NA
Attributes by original data submitter
Sample
source_name
PC-3M cells
differentiation stage
prostate cancer cells
genotype
AR-independent
passages
11
chip antibody
anti-Pol II, #C15200004, lot 001-12, Diagenode
sirna
Ctrl
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Metzger, E., et al. Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation. Nat Cell Biol 10, 53-60 (2008). Libraries were prepared from immunoprecipitated DNA according to standard methods. ChIP-seq libraries were sequenced using a HiSeq 2000 (Illumina) and mapped to the hg19 reference genome using STAR