GSM1377384: Input DNA control TS3.5; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Placenta
Cell type
Trophoblast stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
TS cell
strain background
mixed
cell type
Trophoblast stem (TS) cells
passages
passage 25-40
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP DNA samples were obtained with Native ChIP protocol; briefly, chromatin were fractionated and then subject to Mnase digestion to generate mononucleosomes. Then the mononucleosome fraction was extracted with high salt buffer and H2A.X-containing nucleosomes were isolated and enriched with anti-H2A.X (custom-made) antibodies. Libraries were prepared according to Illumina's standard protocol, with instructions accompanying the DNA Sample Kit