Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Neural

Cell type

Schwann Cells

MeSH Description

Neuroglial cells of the peripheral nervous system which form the insulating myelin sheaths of peripheral axons.

Sample

source_name

H3K4me3 ChIP

cell type

Primary Schwann Cells

treatment

30 minute cAMP at 100 micromolar followed by 3.5 hour washout

antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nascent RNA: Addition of biotinylated Uracil to cell culture followed by biotinylation pulldown of Nascent RNA plus Qiagen miRNAeasy ChIP-Seq: Active Motif ChIP-it NEBNext adapter ligation or SMARTer smRNA-seq adapter ligation kit

Sequencing Platform

instrument_model

Illumina HiSeq 3000

rn6

Number of total reads

50988265

Reads aligned (%)

90.3

Duplicates removed (%)

60.9

Number of peaks

21621 (qval < 1E-05)