Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

hematopoietic stem and progenitor cells (Lin-, cKit+ bone marrow cells)

strain

C57B6/129 hybrid

tissue

Bone marrow

genotype

WT H3

cell type

hematopoietic stem and progenitor cells (Lin-, cKit+ bone marrow cells)

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sheered and fixed samples were lysed and immunoprecipitated. Antibody bound DNA was captured by beads, washed, eluted, reverse-crosslinked and treated with Rnase-A followed by Proteinase-K. ChIP-seq libraries were prepared by end repair, A base addition and ligation of barcoded sequencing adapters (KAPA HyperPrep kit, Roche).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

19350119

Reads aligned (%)

98.6

Duplicates removed (%)

5.8

Number of peaks

129 (qval < 1E-05)

mm9

Number of total reads

19350119

Reads aligned (%)

98.4

Duplicates removed (%)

6.8

Number of peaks

133 (qval < 1E-05)