Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

mESC derived neural cells

NA

NA

Sample

source_name

Neural Progenitor Cells

cell type

R1 ES cell derived neural progenitor cells

chip antibody

none

differentiation

ES cells were differentiated in N2B27 media for 4 days

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and SOX3-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Prep Kit (1003473)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

110111518

Reads aligned (%)

97.6

Duplicates removed (%)

53.5

Number of peaks

645 (qval < 1E-05)

mm9

Number of total reads

110111518

Reads aligned (%)

97.4

Duplicates removed (%)

53.6

Number of peaks

755 (qval < 1E-05)