Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Others
Cell type
AlphaT3-1
NA
NA

Attributes by original data submitter

Sample

source_name
αT3
cell line
alphaT3
genotype/variation
expressing Pax7

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2500 following the manufacturer's protocols to obtain 50bp paired end reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
35317434
Reads aligned (%)
98.5
Duplicates removed (%)
7.0
Number of peaks
218 (qval < 1E-05)

mm9

Number of total reads
35317434
Reads aligned (%)
98.3
Duplicates removed (%)
7.7
Number of peaks
236 (qval < 1E-05)

Base call quality data from DBCLS SRA