GSM3579706: MeCP2 OE 4x ATAC-seq 3; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Neural
Cell type
LUHMES
Tissue
Mesencephalon
Attributes by original data submitter
Sample
source_name
LUHMES-derived neurons
div
Day 9
passage
15-20
cell line
LUHMES
cell type
LUHMES-derived neurons
genotype
MeCP2 OE 11x
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Neurons were scraped from the plate and nuclei were isolated using hypotonic buffer (10 mM Tris-HCl pH7.4, 10 mM NaCl, 3mM MgCl2, 0.1% [v/v] Igepal CA-630) and counted. 50,000 nuclei were resuspended in 50 µl transposition reaction mix containing 2.5 µl Nextera Tn5 Transposase and 2x TD Nextera reaction buffer. The reaction was incubated for 30 min at 37 ºC. DNA was purified by either MinElute PCR kit (Qiagen) or Agencourt AMPure XP (Beckman Coulter) beads and PCR amplified with NEBNext High Fidelity reaction mix (NEB) to generate a sequencing library.