H3K27ac ChIP of wt25Tet3 mESCs replicate 1 PE Read 1
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
Experimental details
H3K27ac ChIP of wt25Tet3 mESCs replicate 1 PE Read 1
strain
mixed C57BL/6J-129V background
dev_stage
embryonic stem cells stage
sex
female
tissue
Mouse embryonic stem cells
cell_type
Embryonic stem cells
collected_by
Temasek Life Sciences laboratory
genotype
Wt25-wild type Tet3 in Tet1,2,3 triple knockout background.
growth_protocol
Tet1, 2 and 3 triple knockout (TKO) mouse embryonic stems (mESC) of mixed C57BL/6J-129V backgrounds (Hu et al., 2014) was cultured in 2i media composed of DMEM GlutaMax 1 mM PD0325901 (StemGent), and 3 mM CHIR99021 (Axon Medchem), 1000 U/ml murine leukemia inhibitory factor (LIF, Chemicon), and 1 × penicillin/streptomycin (PS, Gibco-BRL).
sample_type
Cell culture
Sequenced DNA Library
library_name
ERROR: cannot retrieve data from NCBI: too many requests
library_strategy
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library_source
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library_selection
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library_construction_protocol
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Sequencing Platform
instrument_model
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cycle_sequence
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cycle_count
ERROR: cannot retrieve data from NCBI: too many requests
flow_sequence
ERROR: cannot retrieve data from NCBI: too many requests
flow_count
ERROR: cannot retrieve data from NCBI: too many requests
key_sequence
ERROR: cannot retrieve data from NCBI: too many requests