ChIP-Atlas: SRX5273559

Antigen

Cell type Class: Blood
Cell type: Lymphoma, T-Cell
MeSH Description: A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: ATAC-seq was performed as previously described with minor modifications (Buenrostro et al., Nature Methods, 2013). Fifty thousand cells were pelleted at 550 x g and washed with 1 mL 1x PBS followed by lysis with 50ul lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Accessible chromatin was tagmented with Tn5 transposase (FC-121-1030; Illumina) for 30 minutes in a 37°C waterbath. Tagmented DNA was amplified for 12 cycles of PCR, purified, paired end sequencing (38 bp+37 bp) was performed on a NextSeq 550 following the manufacturer's protocol.