Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Epiblast

NA

NA

Sample

source_name

embryo

tissue

epiblast

genotype

Tet1-/-;Tet2-/-

chip antibody

H3K4me3(home made)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA extraction follows STAR ChIP-seq protocol. The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

18716312

Reads aligned (%)

81.5

Duplicates removed (%)

48.2

Number of peaks

8119 (qval < 1E-05)

mm9

Number of total reads

18716312

Reads aligned (%)

81.4

Duplicates removed (%)

48.2

Number of peaks

8144 (qval < 1E-05)